|
KMID : 1234920140140020055
|
|
Journal of Korean Oriental Association for Study of Obesity
2014 Volume.14 No. 2 p.55 ~ p.62
|
|
|
The Effects of Astragali Radix Extracts on Mitochondrial Function in C2C12 Myotubes
|
|
Song Mi-Young
|
|
|
Abstract
|
|
|
Objective: The prevalence of metabolic syndrome and type 2 diabetes is increasing worldwide. Mitochondrial dysfunction is known to be involved in insulin resistance and obesity, researches have been increasing highly. Astragali Radix extract (ARE) or its main components have been shown to perform comparably to insulin by significantly reducing blood glucose levels in animal models however, the influence on mitochondrial dysfunction are not well understood.
Methods: ARE (0.2, 0.5 and 1.0 mg/ml) or metformin (2.5 mM) were treated in C2C12 after 6 day-differentiation. The expressions of adenosine monophosphate (AMP)-activated protein kinase (AMPK) and phosphorylation AMPK, peroxisome proliferators-activated receptror γ coactivator 1α (PGC1α), nuclear respiratory factors 1 (NRF1), mitochondrial transcription factor (Tfam) and myosin heavy chain were detected with western blotting or polymerase chain reaction analysis. The morphological changes were also investigated.
Results: ARE dose dependently increased phosphorylation of AMPK and respectively activated mRNA expressions of PGC1α, NRF1 and Tfam which are mitochondrial biogenesis regulators. Furthermore, there were some morphologic differences of differentiated cells between ARE treatment and control.
Conclusions: This study suggests that ARE has the potential to increase muscle mitochondrial function by activating AMPK and PGC1α.
|
|
|
KEYWORD
|
|
|
Astragali radix extract, Mitochondria, Myotube, AMP-activated protein kinases, Proliferators-activated receptror γ coactivator 1α
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|